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Analysis of proteomics data

Key functionality

Qlucore Omics Explorer lets the researcher freely examine and analyze data from protemics experiments. For protein analysis the following functionality will be of specific interest

  • Investigate any structure in data using variance filtering combined with PCA.
  • Perform statistical filtering using ANOVA to enhance results.
  • Generate a list of proteins that classifies data based on a selection of statistical tests: F-test, t-tests or regression.
  • Use hierarchical clustering or PCA to indentify subgroups.
  • Work with Variable PCA plots to find correlation and networks among selected proteins.

Platform specific data import

Qlucore Omics Explorer supports data import from various sources. If you have used the analysis software from any of the companies listed below, a brief description of the required import steps is provided. Note that Qlucore Omics Explore expects data to be normalized before input. If you have data from other sources please see the general data import section.

SearchLight® Array Analyst Software (www.aushon.com) is software for image capture and image alignment. Data can be exported to Excel. After export, follow the instructions in the General data import section below.

Ludesi: By using the Ludesi Redfin software (www.ludesi.com), it is easy to export your data into Excel. This is done by pressing the Excel icon at the top right of the user interface. After export, follow the instructions in the General data import section below.

Nonlinear dynamics (www.nonlinear.com) provides the Samespots software. From Samespots the user can export analyzed data into Excel for downstream analysis. See the Samespots user documentation for details. When the file is exported, you can use the description provided in the General Data Import section below.

 

General data import

The Qlucore Import Wizard guides you through import of both data and annotations from many platforms and instruments. You need to start the work with a file with one of the following extensions, .txt, .csv or .tsv. The data should be normallized according to the instrument manufacturers instructions. Open your file from Qlucore Omics Explorer and select the Import Wizard.