Next Generation Sequencing(NGS)
The NGS add-on module enables easy, fast and visual workflows for Next Generation Sequencing (NGS).
Qlucore Omics Explorer is a powerful visualization-based data analysis tool with inbuilt powerful statistics that delivers immediate results and provides instant exploration and visualization. The program supports a broad spectrum of NGS and Omics data. With the NGS module the genomic analysis is enabled.
Analyzing NGS data
Next-generation sequencing (NGS) technologies transforms cancer research by advancing our understanding of cancer pathogenesis and significantly improving the detection, diagnosis, and treatment of these diseases.
The program offers a broad suite of options for what type of Next Generation Sequencing (NGS) data you can analyze and what type of methods you can apply. The NGS module is an add on the base module and all base module functionality is available.
In the center for the NGS data analysis is the the highly interactive and flexible Genome Browser with unique dynamic filtering options. Some examples on available analysis options are:
- Explore RNA-seq data for deeper insights with the synchronized approach, using both expression levels and genomic information. Note that expression based RNA-seq analysis can be done with the base module.
- Call variants with the inbuilt variant caller and visualize content after applying filters on quality and/or type.
- Use external variant data base content to enhance visualization and filter against. Import standard vcf files.
- Perform analysis on ChIP-seq and ATAC-seq data.
- Visualize your NGS data in the Genome Browser in a easy to use environment and control what you see using standard user interface controls – no scripting.
- Filter and select based on the content of standard files such as GTF and BED. Freely select and deselect sample groups.
- Easy to use filtering using check boxes and sliders which regions to include or exclude. Find and identify variants with a few key-presses.
- Analyze regions with a Read Coverage above a slider-defined cutoff or make selections based on the built in variant caller.
- Analyze detected gene fusions using the Gene Fusion Workbench. Examine fusions with the Genome Browser such as directional information of each of the partner genes and the full sequence including the breakpoint. Visualize gene fusions with circle plots.
The innovative pre-processing, which only takes place once, makes the performance requirements manageable and the NGS module is easy to deploy organizational-wide*. Full interactivity is provided for few or hundreds of samples, both for RNA-seq and DNA-seq data. The module accepts standard file formats such as FASTA, BAM, VCF, GTF and BED and results can be exported as reports or high quality images and reports.
Introduction to Qlucore NGS Module
RNA-Seq analysis using Qlucore
Performing gene expression analysis based on RNA sequencing data, in Dilated Cardiomyopathy studies.Read more
Using Qlucore in epigenetics research studies
A range of samples including DNA from patient blood, primary tissue from tumors, and cell lines, are studied.Read more
Qlucore analysis of transcriptomic data
The study includes working with data from more than 400 arrays. Visualization is used in the effort to understand the human growth process.Read more